OrangerV1, France, Analysis, bibRecord, 000289

Effects of nitrogen source, calcium concentration and osmotic stress on protoplasts and protoplast‐derived cell cultures of Pinus Pinaster cotyledons

Identifieur interne : 000289 ( France/Analysis ); précédent : 000288; suivant : 000290

Effects of nitrogen source, calcium concentration and osmotic stress on protoplasts and protoplast‐derived cell cultures of Pinus Pinaster cotyledons

Auteurs : Hélène David [France] ; Edith Jarlet [France] ; Alain David [France]

Source :

Physiologia Plantarum [ 0031-9317 ] ; 1984-07.

RBID : ISTEX:F6399263C43B421F248948F614F7F2D963ACE1A8

Abstract

The experiments described emphasize the effects of several factors crucial to the maintenance of cell divisions leading to increased cell numbers in suspension and colony formation from cotyledon protoplasts of Pinus Pinaster Ait. Osmotic potential of the incubation and culture media are critical. Reducing the osmolality from 680 mOsm kg H2O−1 during protoplast isolation to 610 mOsm kg H2O−1 during washing and culture was essential to achieve a high frequency of cell division. Survival of the cells beyond 3 weeks of culture occurs only if the calcium concentration is decreased from 5.6 mM to 1.5 mM. Glutamine as sole source of nitrogen shortens the lag phase of response of the protoplasts and increases their plating efficiency. After 6 weeks of culture, a combination of low osmolality (225 mOsm kg H2O−1) and high level of glutamine (40 mM) is a prerequisite for obtaining actively growing cell suspensions.

Url:

https://api.istex.fr/document/F6399263C43B421F248948F614F7F2D963ACE1A8/fulltext/pdf

DOI: 10.1111/j.1399-3054.1984.tb06360.x

Affiliations:

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ISTEX:F6399263C43B421F248948F614F7F2D963ACE1A8

Le document en format XML

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<front><div type="abstract" xml:lang="en">The experiments described emphasize the effects of several factors crucial to the maintenance of cell divisions leading to increased cell numbers in suspension and colony formation from cotyledon protoplasts of Pinus Pinaster Ait. Osmotic potential of the incubation and culture media are critical. Reducing the osmolality from 680 mOsm kg H2O−1 during protoplast isolation to 610 mOsm kg H2O−1 during washing and culture was essential to achieve a high frequency of cell division. Survival of the cells beyond 3 weeks of culture occurs only if the calcium concentration is decreased from 5.6 mM to 1.5 mM. Glutamine as sole source of nitrogen shortens the lag phase of response of the protoplasts and increases their plating efficiency. After 6 weeks of culture, a combination of low osmolality (225 mOsm kg H2O−1) and high level of glutamine (40 mM) is a prerequisite for obtaining actively growing cell suspensions.</div>
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Effects of nitrogen source, calcium concentration and osmotic stress on protoplasts and protoplast‐derived cell cultures of Pinus Pinaster cotyledons

Effects of nitrogen source, calcium concentration and osmotic stress on protoplasts and protoplast‐derived cell cultures of Pinus Pinaster cotyledons

Source :

Abstract

Links toward previous steps (curation, corpus...)

Links to Exploration step

Le document en format XML

Pour manipuler ce document sous Unix (Dilib)

Pour mettre un lien sur cette page dans le réseau Wicri